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1、組織不必研磨,不用過(guò)夜消化,直接剪入試劑板,1.5小時(shí)左右提取DNA,DNA長(zhǎng)度50kb~200kb,260/280:1.7~2.0,260/230≥1.5,特別適合于容易降解的動(dòng)物肝臟組織,胰臟組織,海洋水產(chǎn)樣本、昆蟲(chóng)樣品。
2、全血DNA提取得率:人5-12ug/200ul,羊4-10ug/200ul,豬牛5-12ug/200ul,禽類雞鴨50-80ug/20ul,DNA長(zhǎng)度50kb~200kb,260/280:1.7~2.0,260/230≥ 1.5。
3、血清、血漿、細(xì)胞、細(xì)菌、體液、拭子等均可直接提取,DNA完整性高于離心柱試劑盒,得率純度和離心柱試劑盒相當(dāng)。
4、超高靈敏度病毒DNA、RNA提取技術(shù),使你輕松提取血清中微量的病毒DNA、RNA及微量游離核酸。
5、通量大,一次提取32個(gè)樣本,8小時(shí)提取300個(gè)左右樣本,儀器可以申請(qǐng)投放!
iAUTOMAG技術(shù)參數(shù)
★ 處理體積:20uL-1000uL
★ 樣品通量:1-32
★ 磁珠回收效率:>95%
★ 板類型:96孔板
★ 提純靈敏度:102拷貝樣品的陽(yáng)性檢出率 > 95%
★ 提純孔間差:CV < 3%
★ 磁棒:32
★ 磁棒表面磁強(qiáng):大于4000Gs
★ 加熱:可選配加熱模塊,實(shí)現(xiàn)裂解加熱(室溫至 + 80℃)與洗脫加熱(室溫至 + 80℃)
★ 振蕩混合:多模式多檔可調(diào)
★ 磁珠大小:>100nm
★ 操作界面:全中文大屏幕彩色液晶 + 觸控操作
★ 內(nèi)部程序:內(nèi)建15組模式程序
★ 儀器擴(kuò)展接口:USB
★ 程序管理:新建、編輯、刪除,模式程序
★ 殺菌消毒:紫外燈
★ 操作時(shí)間:30-60分鐘/次
★ 網(wǎng)絡(luò)通訊:可擴(kuò)展以太網(wǎng)通訊
實(shí)例:
1. 從組織中提取基因組DNA (牛肌肉組織)
凝膠電泳分析
Lanes 1, 3, 5, 7, 9, 11, 13, 15 were extracted with 20mg of bovine tissues and Lanes 2, 4, 6, 8, 10, 12, 14, 16 were extracted with ddH2O as a negative control in DNA extraction. Note all samples have very similar yields. Purity was also tested and was consistently between 1.9 and 2.0 (not shown).
2. 在細(xì)胞中提取基因組DNA (Hela細(xì)胞系)
瓊脂糖凝膠電泳分析
Lanes 1, 3, 5, 7, 9, 11, 13, 15 were extracted with 1X106 cells of cultured HeLa cell and Lanes 2, 4, 6, 8, 10, 12, 14, 16 were extracted with ddH2O as a negative control in DNA extraction. Note all samples have very similar yields. Purity was also tested and was consistently between 1.8 and 2.0 (not shown).
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